The Anti-PDL1 refers to the anti-human monoclonal antibody with a clone of SP142. It should only be used for research and not in diagnostic procedures. Likewise, it has been tested in humans and comes in sizes of 0.5 ml, 7.0 ml, 0.1 ml, and 1.0 ml.
It is a Type 1 transmembrane protein used in the regulation of humoral immune and cellular responses. It interacts with the receptor PD-1 to provide inhibitory and stimulatory signals and regulates T cell tolerance and activation during tissue allografts, pregnancy, malignant transformation, and autoimmune disease. It is usually expressed in activated B and T cells, antigen presenting cells, placenta, and some tumors, including carcinoma of the colon, lung, and rectum.
Procedure/Application/Controls
The positive control for the IHC application is the placenta with cellular localization in the membrane. Its intended application is Immunohistochemistry (IHC). Likewise, it can be used with paraffin-embedded tissues or formalin-fixed tissues and should be diluted when using a concentrated format, with a ratio of 1:100.
To retrieve the antigens, you will need to boil a tissue section in an EDTA buffer with a pH of 8.0 for 10 minutes and then cool it for 20 minutes. It must incubate at room temperature for at least 10 minutes.
Other Necessary Information
It is best to store the Anti-PDL1 between two and eight degrees Celsius. You should never freeze the antibody. When it is properly stored, it will remain stable until the date located on the label. After the expiration date, it is best to discard the product appropriately. It is tough to determine instability, which means a positive and negative control should both be tested at the same time.
The Anti-PDL1 can be used to check a variety of things, including T-cell activation and more. Visit Spring Bioscience at http://www.springbio.com now to learn more and purchase today.